PDX1 ELISA Kit (Pancreatic and Duodenal Homeobox 1)

Details for Product PDX1 ELISA Kit No. ABIN4884142
  • GSF
  • IDX-1
  • IPF1
  • IUF1
  • MODY4
  • PDX-1
  • STF-1
  • ipf1
  • PDX1
  • IPF-1
  • Ipf1
  • Mody4
  • pdx-1
  • Idx1
  • Stf1
  • zgc:100874
  • XlHbox-8
  • xlhbox8
  • pancreatic and duodenal homeobox 1
  • pancreatic and duodenal homeobox 1 S homeolog
  • PDX1
  • pdx1
  • Pdx1
  • pdx1.S
Kits with alternative reactivity to:
Detection Method
Method Type
Sandwich ELISA
Purpose Custom Human PDX-1 (IPF1) ELISA Kit.
Sample Type Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This ELISA antibody pair recognizes Human PDX-1. Other species not yet determined.
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Plasmids, Primers & others Plasmids, Primers & others PDX1 products on genomics-online (e.g. as negative or positive controls)
Alternative Name PDX-1 (PDX1 ELISA Kit Abstract)
Gene ID 3651
UniProt P52945
Pathways Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location
Sample Volume 100 μL
Plate Pre-coated
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months