CD11b ELISA Kit

Catalog No. ABIN2043771

This Human CD11b ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CD11b.

Quick Overview for CD11b ELISA Kit (ABIN2043771)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CD11b ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.125 ng/mL

Application Details

Plate: Pre-coated

Protocol: Integrin Alpha M (ITGaM) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha M (ITGaM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha M (ITGaM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha M (ITGaM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Integrin Alpha M (ITGaM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation