CCL15 ELISA Kit

Catalog No. ABIN2043905

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CCL15.

Quick Overview for CCL15 ELISA Kit (ABIN2043905)

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details CCL15 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Brand: BioAssay™

Sensitivity: < 5.3 pg/mL

Application Details

Plate: Pre-coated

Protocol: Macrophage Inflammatory Protein 5 (MIP5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 5 (MIP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 5 (MIP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 5 (MIP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Macrophage Inflammatory Protein 5 (MIP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CCL15

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Alternative Name: Macrophage Inflammatory Protein 5 (MIP5)