NDRG2 ELISA Kit

Catalog No. ABIN2044031

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat NDRG2.

Quick Overview for NDRG2 ELISA Kit (ABIN2044031)

Target: NDRG2 (NDRG Family Member 2 (NDRG2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details NDRG2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.141 ng/mL

Application Details

Plate: Pre-coated

Protocol: N-myc Downstream Regulated Gene 2 (NDRG2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-myc Downstream Regulated Gene 2 (NDRG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-myc Downstream Regulated Gene 2 (NDRG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-myc Downstream Regulated Gene 2 (NDRG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of N-myc Downstream Regulated Gene 2 (NDRG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NDRG2

Target: NDRG2 (NDRG Family Member 2 (NDRG2))

Alternative Name: N-Myc Downstream Regulated Gene 2 (NDRG2)