MPZ ELISA Kit

Catalog No. ABIN2044355

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MPZ.

Quick Overview for MPZ ELISA Kit (ABIN2044355)

Target: MPZ (Myelin Protein Zero (MPZ))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details MPZ ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Brand: BioAssay™

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: Protein Zero, Myelin (MPZ) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Zero, Myelin (MPZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Zero, Myelin (MPZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Zero, Myelin (MPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Zero, Myelin (MPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MPZ

Target: MPZ (Myelin Protein Zero (MPZ))

Alternative Name: Protein Zero, Myelin (MPZ)