CCL17 ELISA Kit

Catalog No. ABIN2044665

This Human CCL17 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CCL17.

Quick Overview for CCL17 ELISA Kit (ABIN2044665)

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Application: ELISA

Product Details CCL17 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Brand: BioAssay™

Sensitivity: < 10.5 pg/mL

Application Details

Plate: Pre-coated

Protocol: Thymus Activation Regulated Chemokine (TARC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymus Activation Regulated Chemokine (TARC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymus Activation Regulated Chemokine (TARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymus Activation Regulated Chemokine (TARC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Thymus Activation Regulated Chemokine (TARC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CCL17

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: Thymus Activation Regulated Chemokine (TARC)