NAMPT ELISA Kit

Catalog No. ABIN2044797

This Guinea Pig NAMPT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Guinea Pig NAMPT.

Quick Overview for NAMPT ELISA Kit (ABIN2044797)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Guinea Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details NAMPT ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Brand: BioAssay™

Sensitivity: < 0.52 ng/mL

Application Details

Plate: Pre-coated

Protocol: Visfatin (VF) BioAssay-ELISA Kit (Guinea Pig) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Visfatin (VF)