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Vitamin D-Binding Protein ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat Vitamin D-Binding Protein.

Catalog No. ABIN2044799

Quick Overview for Vitamin D-Binding Protein ELISA Kit (ABIN2044799)

Target

See all Vitamin D-Binding Protein (GC) ELISA Kits

Vitamin D-Binding Protein (GC)

Reactivity

15
12
8
4
2
2
2
2
2
2
1
1
1

Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

12.5-800 ng/mL

Application

ELISA

Discover our top product GC ELISA Kit

Minimum Detection Limit

12.5 ng/mL

Sensitivity

< 5.57 ng/mL
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GC Reactivity: Rat Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate

Vitamin D-Binding Protein (GC) ELISA Kit
GC Reactivity: Mouse Colorimetric Sandwich ELISA 3.906 ng/mL - 250 ng/mL Plasma, Serum, Tissue Homogenate

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GC Reactivity: Mouse Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum

Vitamin D-Binding Protein (GC) ELISA Kit
GC Reactivity: Cow Colorimetric Competition ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate

Vitamin D-Binding Protein (GC) ELISA Kit
GC Reactivity: Human Colorimetric Sandwich ELISA 3.91 ng/mL - 250 ng/mL Plasma, Serum

Vitamin D-Binding Protein (GC) ELISA Kit
GC Reactivity: Rat Colorimetric Sandwich ELISA 15.63 ng/mL - 1000 ng/mL Plasma, Serum

Plate

Pre-coated

Protocol

Vitamin D Binding Protein (DBP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein (DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vitamin D Binding Protein (DBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions

For Research Use only
Storage

4 °C/-20 °C
Target See all Vitamin D-Binding Protein (GC) ELISA Kits

Vitamin D-Binding Protein (GC)

Alternative Name

Vitamin D Binding Protein (DBP)

Pathways

Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process

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