IL1A ELISA Kit

Catalog No. ABIN2111981

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL1A.

Quick Overview for IL1A ELISA Kit (ABIN2111981)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma

Product Details IL1A ELISA Kit

Minimum Detection Limit: 15.6 ng/mL

Analytical Method: Quantitative

Sensitivity: 9 pg/mL

Components: Mouse IL-1alpha Standard, lyophilized, 1x1ug
Detection Antibody, 1x20 µL
HRP Labeled Streptavidin, lyophilized, 1x2ug
Wash Buffer 10X, 2x30 mL
ELISA Buffer 10X, 2x30 mL
TMB Substrate Solution, 1x10 mL
Stop Solution, 1x10 mL
Plate coated with mIL-1alpha antibody, 6x16 well strips
Plate covers, 2x plastic film
Silica gel, 2x minibags

Application Details

Plate: Pre-coated

Protocol: This BioAssay-is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of mouse IL-1alpha in cell culture supernatants, serum and plasma. A monoclonal antibody specific for IL-1alpha has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, IL-1alpha is recognized by the addition of a biotinylated monoclonal antibody specific for IL-1alpha (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3-,5,5--tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of IL-1alpha in the samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1alpha

Background: The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not re- quired for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints