11,12-EET/DHET ELISA Kit

Catalog No. ABIN2648461

Chemical ELISA Kit Colorimetric assay for quantification of Chemical .

Quick Overview for 11,12-EET/DHET ELISA Kit (ABIN2648461)

Target: 11,12-EET/DHET

Reactivity: Chemical

Detection Method: Colorimetric

Application: ELISA

Sample Type: Cell Culture Cells, Cell Culture Supernatant, Plasma, Serum

Product Details

Sensitivity: 10 pg/mL

Characteristics: It is well known that arachidonic acid (AA) will be converted to EET by P450 arachidonic acid epoxygenase (AA epoxygenase) and EET will be converted to DHET by soluble epoxide hydrolase (sEH) in vivo. Cytochrome P450 2J2 (CYP2J2) is a predominant human AA epoxygenase that produces all four EETs. The EET/DHET ELISA Assay kit can be used to measure EET levels in cultured cells which express sEH (1).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 2.5 h

Protocol: 11,12-EET/DHET ELISA Assay kit is intended for the quantitative determination of 11,12-EET/DHET in biological samples by enzyme linked immunoassay (ELISA). 11,12-EET/DHET ELISA Assay kit is for research use only and not to be used in diagnostic procedures

Restrictions: For Research Use only

Handling

Storage: -20 °C

Target Details

Target: 11,12-EET/DHET