ACTH ELISA Kit

Catalog No. ABIN2648478

This Chemical ACTH ELISA Kit is a Colorimetric ELISA Kit designed to quantify Chemical ACTH.

Quick Overview for ACTH ELISA Kit (ABIN2648478)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Chemical

Detection Method: Colorimetric

Application: ELISA

Sample Type: Plasma

Product Details ACTH ELISA Kit

Sensitivity: .4 pg/ml

Characteristics: Human Adrenocorticotrophic Hormone (ACTH) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Adrenocorticotrophic Hormone (ACTH) levels in plasma. Human Adrenocorticotrophic Hormone (ACTH) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 200 μL

Assay Time: 2.5 h

Protocol: Human Adrenocorticotrophic Hormone (ACTH) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human ACTH in EDTA-plasma sample. The assay utilizes the two-site "sandwich" technique with selected antibodies that bind to N-terminal and C-terminal epitopes of ACTH. Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to the N-terminal of human ACTH. Immediately, a horseradish peroxidase (HRP) conjugated anti C-terminal of human ACTH antibody is added to each well. After the first incubation period, a "sandwich" of solid-phase monoclonal antibody - human ACTH - HRP conjugated antibody" is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and the absorbances are then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human ACTH in the test sample. A standard curve is generated by plotting the absorbance versus the respective human ACTH concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human ACTH in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Target Type: Hormone

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity