PRO-ANP ELISA Kit

Catalog No. ABIN2648514

This Chemical PRO-ANP ELISA Kit is a Colorimetric ELISA Kit designed to quantify Chemical PRO-ANP.

Quick Overview for PRO-ANP ELISA Kit (ABIN2648514)

Target: PRO-ANP (Pro-Atrial Natriuretic Peptide (PRO-ANP))

Binding Specificity: N-Term

Reactivity: Chemical

Detection Method: Colorimetric

Application: ELISA

Sample Type: Plasma, Urine, Serum, Cell Culture Supernatant

Product Details PRO-ANP ELISA Kit

Sensitivity: 0.05 nmol/L

Characteristics: Atrial natriuretic peptide is synthesized in atrial myocytes and is stored in secretory granules as a 126 amino acid prohormone. The most important stimulus for the release of the hormone into circulation is stretch of the myocyte fibres. On release the prohormone is split into equimolar amounts of the highly biologically active proANP (99-126), also known as a-ANP, and the N-terminal part proANP (1-98). a-ANP is rapidly cleared from the circulation with a half-life of 3-4 minutes. proANP (1-98) has a much longer half-life (60-120 min) which leads to significantly higher concentrations in blood compared to a-ANP. Thus, circulating levels of proANP (1-98) are less sensitive to the pulsatile secretion of ANP and may better reflect chronic levels of ANP secretion than the rapidly fluctuating levels of a-ANP. proANP is discussed as valuable marker for e.g. sepsis (Increased plasma levels of NT-proANP and NT-proBNP as markers of cardiac dysfunction in septic patients. Hoffmann U. et al., Clin. Lab. 2005,51 (7-8):373-9), or risk stratification in heart failure (Neurohormonal risk stratification for sudden death and death owing to progressive heart failure in chronic heart failure. Berger R. et al, European Journal of Clinical Investigation, 2005, 35 (1), 24-31)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 10 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: proANP (1-98) ELISA Assay KIT(enzyme-linked immunoassay kit) is intended for the quantitative determination of human, mouse, or rat proANP(1-98) in EDTA PLASMA, HEPARIN PLASMA, serum, URINE or CELL CULTURE SUPERNATANTS. proANP (1-98) ELISA Assay KIT is manufactured by Biomedica Gruppe (www.bmgrp.com) and is for research use only, not to be used in diagnostic procedures.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for PRO-ANP

Target: PRO-ANP (Pro-Atrial Natriuretic Peptide (PRO-ANP))

Alternative Name: NT-ProANP