G-CSF ELISA Kit
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- Target See all G-CSF (CSF3) ELISA Kits
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Purpose
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Granulocyte Colony Stimulating Factor (G-CSF) concentrations in cell culture supernates, serum, and plasma.
- Sample Type
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensitivity
- 4 pg/ml
- Characteristics
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
- Top Product
- Discover our top product CSF3 ELISA Kit
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Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA Kit
CSF3 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 Reactivity: Mouse Colorimetric Sandwich ELISA 0.5-150 pg/mL Cell Culture Supernatant, Plasma, Serum
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 Reactivity: Rat Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 Reactivity: Rabbit Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 Reactivity: Rat Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 Reactivity: Mouse AA 31-208 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 Reactivity: Human AA 31-204 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 3.5 h
- Plate
- Pre-coated
- Protocol
- Human Granulocyte Colony Stimulating Factor (G-CSF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for G-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for G-CSF is added to the wells and binds to the combination of capture antibody- G-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of G-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven G-CSF standard dilutions and G-CSF sample concentration determined.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all G-CSF (CSF3) ELISA Kits
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- Alternative Name
- G-CSF (CSF3 Products)
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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