CXCL12 ELISA Kit
Quick Overview for CXCL12 ELISA Kit (ABIN2648568)
Target
See all CXCL12 ELISA KitsReactivity
Detection Method
Application
Sample Type
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Sensitivity
- 0.31 ng/ml
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Characteristics
- SDF-1 Alpha (CXCL12) is a CXC chemokine [1]. The small basic protein (ca. 8 kDa) has no glycosylation site in its sequence [2]. The homology properties of SDF-1 Alpha are unique among the chemokines. It has a high degree of conservation within the mammalians, with 99 % identity between the human and the murine SDF-1 Alpha sequences. But it shows a weak homology to the other CXC chemokines and the SDF-1 gene is on chromosome 10, whereas the other chemokine genes are on chromosome 4 and 17 [3].
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Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA Kit
CXCL12 Reactivity: Human Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA KitCXCL12 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA KitCXCL12 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA KitHigh Sensitivity CXCL12 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA KitCXCL12 Reactivity: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) ELISA KitCXCL12 Reactivity: Rabbit Colorimetric Sandwich ELISA 0.57-10 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 50 μL
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Assay Time
- 2.5 h
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Plate
- Pre-coated
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Protocol
- The biotinylated antibody of the CXCL 12 / SDF-1 Alpha ELISA Assay kit binds to the streptavidin coated microtiterplate. The SDF-1 α of the standards/samples binds to this antibody and is detected by the primary antibody. The biotinylated antibody, the primary antibody and the standards/samples are incubated on the microtiterplate altogether at the same time. The primary antibody, which is bound to SDF-1 Alpha, is then detected by a secondary peroxidase-conjugated antibody. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of SDF-1 Alpha.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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Alternative Name
- CXCL 12/SDF-1 alpha
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Pathways
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
Target See all CXCL12 ELISA Kits
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