Dopamine ELISA Kit

Catalog No. ABIN2648625

This Chemical Dopamine ELISA Kit is a Colorimetric ELISA Kit designed to quantify Chemical Dopamine.

Quick Overview for Dopamine ELISA Kit (ABIN2648625)

Target: Dopamine (DA)

Reactivity: Chemical

Detection Method: Colorimetric

Application: ELISA

Sample Type: Cell Culture Cells, Plasma, Tissue Homogenate

Product Details Dopamine ELISA Kit

Purpose: The Dopamine High Sensitive ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of noradrenaline in biological samples including serum, plasma, tissue, and cell culture samples.

Analytical Method: Quantitative

Specificity: High Sensitive ELISA

Sensitivity: 5.9 pg/mL

Characteristics: The Dopamine High Sensitive ELISA Assay kit is for research use only and should not be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Plate: Pre-coated

Protocol: The competitive Dopamine High Sensitive ELISA kit uses the microtitre plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated catecholamine from the sample and solid phase bound catecholamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase catecholamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase catecholamine is inversely proportional to the catecholamine concentration of the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Dopamine

Target: Dopamine (DA)

Alternative Name: Dopamine

Target Type: Chemical