Anti-Gliadin IgA ELISA Kit

Catalog No. ABIN2648661

This Human ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human .

Quick Overview for Anti-Gliadin IgA ELISA Kit (ABIN2648661)

Target: Anti-Gliadin IgA

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Fecal

Product Details

Purpose: Anti-Gliadin sIgA / IgA ELISA kit allows for an easy, rapid and precise quantitative determination of the secretory IgA and IgA gliadin antibodies in stool. The kit includes all reagents ready to use for preparation of the samples.

Analytical Method: Quantitative

Sensitivity: 1.3 mU/g

Characteristics: Celiac disease is a chronic illness of the small intestinal mucous membrane. It is caused by an intolerance against gluten, which is found in many cereals. The intake of gluten-containing food leads to inflammation of the small intestinal mucous membrane. The resorption of nutrients is therefore reduced. The symptoms of the disease are reduction of weight, diarrhoea, vomiting, anorexia and tiredness. The growth in children is reduced. The only therapeutic treatment is a gluten-free diet. An untreated celiac disease has been known to increase the risk of non-Hodgkin-lymphoma and colon cancer. In five to ten percent of the patients, celiac disease is associated with diabetes mellitus type 1. Women are more often affected than men. The outcome of the disease is pronounced during infancy and in an age between 30 and 40 years.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 mg

Assay Time: 2 h

Protocol: The Anti-Gliadin sIgA / IgA ELISA kit determines human anti-gliadin sIgA / IgA antibodies according to the "sandwich"-principle. Anti-gliadin antibodies in sample, standard and controls bind to gliadin, which is coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm in a microtiter plate reader. The anti-gliadin concentration can be calculated from the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Anti-Gliadin IgA

Alternative Name: Anti-Gliadin sIgA / IgA

Target Type: Antibody