IGFBP4 ELISA Kit
Quick Overview for IGFBP4 ELISA Kit (ABIN2648687)
Target
See all IGFBP4 ELISA KitsReactivity
Detection Method
Application
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Purpose
- IGFBP-4 ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) in biological fluids.
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Analytical Method
- Quantitative
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Sensitivity
- 0.625 ng/ml
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Characteristics
- The IGFBP-4 ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
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Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit
IGFBP4 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Chicken Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Cow Colorimetric Competition ELISA 74.07 pg/mL - 6000 pg/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Human AA 22-258 Colorimetric Sandwich ELISA 500-32000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Human Colorimetric Sandwich ELISA 500 pg/mL - 32000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA KitIGFBP4 Reactivity: Goat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 50 μL
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Assay Time
- 2 h
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Plate
- Pre-coated
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Protocol
- The biotinylated antibody binds to the streptavidin coated microtiter plate. The IGFBP-4 of the standards/samples binds to this antibody. The biotinylated antibody and the standards/ samples are incubated on the microtiter plate at the same time. After a washing step the bound IGFBP-4, is then detected by a horseradish peroxidase-conjugated anti-IGFBP-4 antibody. After a second washing step the conjugate is added and incubated. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of IGFBP-4.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
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Alternative Name
- IGFBP-4
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Pathways
- WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
Target See all IGFBP4 ELISA Kits
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