Kallikrein 7 ELISA Kit

Catalog No. ABIN2648745

This Human Kallikrein 7 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Kallikrein 7.

Quick Overview for Kallikrein 7 ELISA Kit (ABIN2648745)

Target: Kallikrein 7 (KLK7)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum

Product Details Kallikrein 7 ELISA Kit

Sensitivity: 2 ng/ml

Characteristics: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK7 is one of the fifteen kallikrein subfamily members. The KLK7 enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 2.5 h

Plate: Pre-coated

Protocol: The KLK7 (SCCE) ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact KLK7 Molecule for solid phase immobilization (on the microtiter wells). Standards, calibrators, and patient samples are incubated with the solid phase antibody on the plate. Wells are then washed and incubated with a biotin conjugated anti-KLK7 monoclonal antibody. After a second wash Streptavidin conjugated to HRP is added as a reporting agent. Excess streptavidin-HRP is then washed off and a solution of TMB Reagent is added and incubated resulting in the development of a blue color if KLK7 is present. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of KLK7 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Kallikrein 7

Target: Kallikrein 7 (KLK7)

Alternative Name: Kallikrein-Related Peptidase 7 (KLK7)

Pathways: Complement System