Kallikrein 8 ELISA Kit
Quick Overview for Kallikrein 8 ELISA Kit (ABIN2648746)
Target
See all Kallikrein 8 (KLK8) ELISA KitsReactivity
Detection Method
Application
Sample Type
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Sensitivity
- 1 ng/ml
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Characteristics
- Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK8 is one of the fifteen kallikrein subfamily member. The KLK8 enzyme exhibits distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.
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Kallikrein 8 (KLK8) ELISA Kit
KLK8 Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Kallikrein 8 (KLK8) ELISA KitKLK8 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Kallikrein 8 (KLK8) ELISA KitKLK8 Reactivity: Human Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma, Serum
Kallikrein 8 (KLK8) ELISA KitKLK8 Reactivity: Human AA 29-260 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 2.5 h
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Plate
- Pre-coated
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Protocol
- The KLK8 (TADG-14) ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact KLK8 Molecule for solid phase immobilization (on the microtiter wells). Standards, calibrators, and patient samples are incubated with the solid phase antibody on the plate. Wells are then washed and incubated with an anti-KLK8polyclonal antibody. After a second wash Goat anti-Rabbit antibody conjugated to HRP is added as a reporting agent. Excess Goat anti-Rabbit -HRP is then washed off and a solution of TMB Reagent is added and incubated resulting in the development of a blue color if KLK8 is present. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of KLK8 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Kallikrein 8 (KLK8)
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Alternative Name
- Kallikrein-Related Peptidase 8 (KLK8)
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Pathways
- Complement System
Target See all Kallikrein 8 (KLK8) ELISA Kits
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