IL-17 ELISA Kit

Catalog No. ABIN2648755

Human IL-17 ELISA Kit Colorimetric assay for quantification of Human IL-17.

Quick Overview for IL-17 ELISA Kit (ABIN2648755)

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma, Cell Culture Cells

Product Details IL-17 ELISA Kit

Purpose: Human Interleukin 17 (IL-17) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 17 (IL-17) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensitivity: 7 pg/ml

Characteristics: Human Interleukin 17 (IL-17) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Human Interleukin 17 (IL-17) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-17 is added to the wells and binds to the combination of capture antibody-IL-17 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-17 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-17 standard dilutions and IL-17 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-17

Target: IL-17 (IL17) (Interleukin 17 (IL17))

Alternative Name: IL-17