IL-22 ELISA Kit
Quick Overview for IL-22 ELISA Kit (ABIN2648761)
Target
See all IL-22 (IL22) ELISA KitsReactivity
Detection Method
Application
Sample Type
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Purpose
- Human Interleukin 22 (IL-22) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 22 (IL-22) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensitivity
- 15 ng/ml
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Characteristics
- Human Interleukin 22 (IL-22) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interleukin 22 (IL22) ELISA Kit
IL22 Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Plasma, Serum, Urine
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 22 (IL22) ELISA KitSmall Sample IL22 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Pig Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Human AA 34-179 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Human Colorimetric Sandwich ELISA 31.25-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 22 (IL22) ELISA KitIL22 Reactivity: Pig Colorimetric Sandwich ELISA 20-6.000 pg/mL Cell Culture Supernatant, Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 3.5 h
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Plate
- Pre-coated
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Protocol
- Human Interleukin 22 (IL-22) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-22 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-22 is added to the wells and binds to the combination of capture antibody- IL-22 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-22 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-22 standard dilutions and IL-22 sample concentration determined.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- IL-22 (IL22) (Interleukin 22 (IL22))
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Alternative Name
- IL-22
Target See all IL-22 (IL22) ELISA Kits
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