ENO2/NSE ELISA Kit

Catalog No. ABIN2648800

Human ENO2/NSE ELISA Kit Colorimetric assay for quantification of Human ENO2/NSE.

Quick Overview for ENO2/NSE ELISA Kit (ABIN2648800)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum

Product Details ENO2/NSE ELISA Kit

Sensitivity: 1.2 ng/ml

Characteristics: Human Neuron Specific Enolase (NSE) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human neuron specific enolase (NSE) levels in serum. Human Neuron Specific (NSE) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 10 μL

Assay Time: 1 h

Protocol: Human Neuron Specific Enolase (NSE) ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human NSE in serum sample. The assay utilizes the two-site "sandwich" technique with two selected monoclonal antibodies that bind to different epitopes of the γ-subunit of the enzyme. Assay standards, controls and patient samples are added directly to wells of microplate that is coated with a streptavidin. Subsequently, a mixture of a biotinylated NSE specific monoclonal antibody and a horseradish peroxidase (HRP) labeled NSE specific monoclonal antibody is added to each microtiter well. After the first incubation a "sandwich" immunocomplex of "streptavidin-biotin-monoclonal antibody - human NSE - monoclonal antibody-HRP" is formed. The unbound monoclonal antibodies are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the NSE on the wall of the microtiter well is directly proportional to the amount of NSE in the sample. A standard curve is generated by plotting the absorbance versus the respective human NSE concentration for each standard on point-to-point, cubical scales or 4 parameter curve fit. The concentration of human NSE in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: Neuron Enolase (NSE)