Osteocalcin ELISA Kit

Catalog No. ABIN2648801

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Osteocalcin.

Quick Overview for Osteocalcin ELISA Kit (ABIN2648801)

Target: Osteocalcin (BGLAP)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum, Plasma

Product Details Osteocalcin ELISA Kit

Sensitivity: .31 ng/ml

Characteristics: Human Osteocalcin ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of both human osteocalcin (1-49) and osteocalcin (1-43) (also referred as N-terminal & mid-regional osteocalcin) levels in test samples). Human Osteocalcin ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 25 μL

Assay Time: 1.5 h

Protocol: Human Osteocalcin ELISA Assay Kit is designed, developed and produced for the quantitative measurement of of human osteocalcin (1-49) and (1-43) in serum or plasma sample. The assay utilizes the two-site "sandwich" technique with two selected antibodies that bind to different epitopes of human osteocalcin. Assay standards, controls and samples are added directly to wells of a microtiter plate that is coated with streptavidin. Subsequently, a mixture of biotinylated human osteocalcin N-terminal region specific polyclonal antibody and a peroxidase labeled human osteocalcin 20 - 43 region specific monoclonal antibody is added to each well. After the first incubation period, a "sandwich" of "biotinylated antibody - human osteocalcin - HRP-monoclonal antibody" is formed and this immunocomplex is also captured to the wall of microtiter plate via a streptavidin-biotin affinity binding. The unbound monoclonal antibodies and buffer matrix are removed in the subsequent washing step. A substrate solution in a timed reaction is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human osteocalcin in a test sample. A standard curve is generated by plotting the absorbance versus the respective human osteocalcin concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of human osteocalcin in test samples is determined directly from this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: Osteocalcin