SLPI ELISA Kit
Quick Overview for SLPI ELISA Kit (ABIN2648824)
Target
See all SLPI ELISA KitsReactivity
Detection Method
Application
Sample Type
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Sensitivity
- 1 ng/ml
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Characteristics
- The Secretory Leukocyte Peptidase Inhibitor Protein (SLPI) ELISA assay kit is a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes. The SLPI (ALP) protein is also thought to have broad-spectrum antibiotic activity.
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Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit
SLPI Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA KitSLPI Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA KitSLPI Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA KitSLPI Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA KitSLPI Reactivity: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 2.5 h
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Plate
- Pre-coated
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Protocol
- The Secretory Leukocyte Peptidase Inhibitor Protein (SLPI) ELISA assay kit is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact SLPI molecule for solid phase immobilization (on the microtiter wells). Standards, calibrators, and patient samples are incubated with the solid phase antibody on the plate. Wells are then washed and incubated with a HRP conjugated anti-SLPI monoclonal antibody. Excess anti-SLPI-HRP antibody is then washed off and a solution of TMB Reagent is added and incubated resulting in the development of a blue color if SLPI is present. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of SLPI is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- SLPI (Secretory Leukocyte Peptidase Inhibitor (SLPI))
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Alternative Name
- Secretory Leukocyte Peptidase Inhibitor Protein (SLPI)
Target See all SLPI ELISA Kits
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