Thymic Stromal Lymphopoietin ELISA Kit
Quick Overview for Thymic Stromal Lymphopoietin ELISA Kit (ABIN2648846)
Target
See all Thymic Stromal Lymphopoietin (TSLP) ELISA KitsReactivity
Detection Method
Application
Sample Type
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Purpose
- Human Thymic Stromal Lymphopoietin (TSLP) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Thymic Stromal Lymphopoietin (TSLP) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensitivity
- 2 pg/ml
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Characteristics
- Human Thymic Stromal Lymphopoietin (TSLP) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Thymic Stromal Lymphopoietin (TSLP) ELISA Kit
TSLP Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitHigh Sensitivity TSLP Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Monkey Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Human AA 29-159 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Mouse AA 20-140 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Mouse Colorimetric Sandwich ELISA 0.02-1.0 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Human Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Thymic Stromal Lymphopoietin (TSLP) ELISA KitTSLP Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Sample Volume
- 100 μL
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Assay Time
- 3.5 h
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Plate
- Pre-coated
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Protocol
- Human Thymic Stromal Lymphopoietin (TSLP) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TSLP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSLP present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TSLP is added to the wells and binds to the combination of capture antibody-TSLP in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TSLP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TSLP standard dilutions and TSLP sample concentration determined.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- Thymic Stromal Lymphopoietin (TSLP)
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Alternative Name
- TSLP
Target See all Thymic Stromal Lymphopoietin (TSLP) ELISA Kits
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