Quick Overview for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213)
Target
See all Annexin V (ANXA5) KitsReactivity
Application
-
-
Conjugate
- FITC
-
Characteristics
- FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
-
Components
- FITC Annexin V Apoptosis Detection Kit with PI
-
-
Annexin A5 (ANXA5) ELISA Kit
ANXA5 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 Reactivity: Mouse Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Annexin A5 (ANXA5) ELISA KitANXA5 Reactivity: Monkey Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 Reactivity: Chicken Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 Reactivity: Horse Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Annexin A5 (ANXA5) ELISA KitANXA5 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Restrictions
- For Research Use only
-
-
-
Precaution of Use
- Caution: Propidium Iodide Solution is toxigenic and mutagenic, handle with care.
-
Handling Advice
- Do not freeze.
-
Storage
- 4 °C
-
Storage Comment
- Store between 2°C and 8°C.
-
-
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
-
Alternative Name
- Annexin V
Target
-
