IL36A/IL1F6 ELISA Kit

Catalog No. ABIN2703145

This Human IL36A/IL1F6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL36A/IL1F6.

Quick Overview for IL36A/IL1F6 ELISA Kit (ABIN2703145)

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.8-200 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details IL36A/IL1F6 ELISA Kit

Minimum Detection Limit: 0.8 ng/mL

Purpose: Human IL-36 alpha (IL-1 F6) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1, IL-1F2, IL-1F3, IL-1F4, IL-1F5, IL-1F7, IL-1F8, IL-1F9, IL-1F10, IL-1F11.

Sensitivity: 0.8 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Sample Preparation:

Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IL36A/IL1F6

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Alternative Name: IL-36 alpha

Background: Gene Names: IL36A FIL1E IL1E IL1F6
Protein names: Interleukin-36 alpha (FIL1 epsilon) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 6) (IL-1F6)

Gene ID: 27179

UniProt: Q9UHA7

Pathways: Cancer Immune Checkpoints