COL18A1 ELISA Kit

Catalog No. ABIN2859242

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse COL18A1. There are 3 publications available.

Quick Overview for COL18A1 ELISA Kit (ABIN2859242)

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Binding Specificity: AA 1591-1774

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details COL18A1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Endostatin

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: H1591-K1774

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: H1591-K1774

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the multiplexin collagen family.

Tissue Specificity: Expressed in liver, kidney, lung, skeletal muscle and testis. .

Plate: Pre-coated

Protocol: mouse Endostatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endostatin has been precoated onto 96-well plates. Standards(NSO, H1591-K1774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endostatin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Endostatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Endostatin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 0.8, Standard deviation: 0.052, CV(%): 6.5
• Sample 2: n=16, Mean(ng/ml): 2.14, Standard deviation: 0.135, CV(%): 6.3
• Sample 3: n=16, Mean(ng/ml): 4.25, Standard deviation: 0.179, CV(%): 4.2,
• Sample 1: n=24, Mean(ng/ml): 0.84, Standard deviation: 0.06, CV(%): 7.1
• Sample 2: n=24, Mean(ng/ml): 2.23, Standard deviation: 0.147, CV(%): 6.6
• Sample 3: n=24, Mean(ng/ml): 4.37, Standard deviation: 0.253, CV(%): 5.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for COL18A1 ELISA Kit (ABIN2859242)

Guo, Wu, Cen, Li, Kong, Zhou: "IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis." in: Virology journal, Vol. 11, Issue 1, pp. 2511, (2014) (PubMed).

Fu, Zhao, Wu, Wu, Xu, Liu, Tong: "Therapeutic mechanisms of Tongmai Dasheng Tablet on tripterygium glycosides induced rat model for premature ovarian failure." in: Journal of ethnopharmacology, Vol. 139, Issue 1, pp. 26-33, (2011) (PubMed).

Zhang, Qu, Cui, Guo, Zhang, Ma, Sun: "Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice." in: Cancer biology & therapy, Vol. 8, Issue 5, pp. 466-73, (2009) (PubMed).

Target Details for COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Alternative Name: Endostatin

Target Type: Chemical

Background:

Protein Function: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.

Background: Endostatin is a naturally-occurring 20- kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Synonyms: Collagen alpha-1(XVIII) chain,Endostatin,Col18a1,

Full Gene Name: Collagen alpha-1(XVIII) chain

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 12822

UniProt: P39061