SERPINC1 ELISA Kit

Catalog No. ABIN2859247

Human SERPINC1 ELISA Kit Colorimetric assay for quantification of Human SERPINC1.

Quick Overview for SERPINC1 ELISA Kit (ABIN2859247)

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Binding Specificity: AA 33-464

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20.000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Product Details SERPINC1 ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Serpin C1/Antithrombin-III

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: NSO, H33-K464

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: H33-K464

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Found in plasma.

Plate: Pre-coated

Protocol: human Serpin C1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin C1 has been precoated onto 96-well plates. Standards (NSO, H33-K464) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin C1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Serpin C1 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Serpin C1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Serpin C1 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.05, Standard deviation: 0.064, CV(%): 6.1
• Sample 2: n=16, Mean(ng/ml): 3.42, Standard deviation: 0.194, CV(%): 5.7
• Sample 3: n=16, Mean(ng/ml): 8.67, Standard deviation: 0.546, CV(%): 6.3,
• Sample 1: n=24, Mean(ng/ml): 1.32, Standard deviation: 0.088, CV(%): 6.7
• Sample 2: n=24, Mean(ng/ml): 3.74, Standard deviation: 0.239, CV(%): 6.4
• Sample 3: n=24, Mean(ng/ml): 8.23, Standard deviation: 0.543, CV(%): 6.6

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for SERPINC1

Target: SERPINC1 (Serpin Family C Member 1 (SERPINC1))

Alternative Name: SERPINC1

Background:

Protein Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. .

Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22 % of the intron sequence) than in the genome as a whole (about 5 %).

Synonyms: Antithrombin-III,ATIII,Serpin C1,SERPINC1,AT3,PRO0309,

Full Gene Name: Antithrombin-III

Cellular Localisation: Secreted, extracellular space.

Gene ID: 462

UniProt: P01008