Histone 3 ELISA Kit

Catalog No. ABIN2866242

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Histone 3.

Quick Overview for Histone 3 ELISA Kit (ABIN2866242)

Target: Histone 3 (H3) (Histone H3 (H3))

Binding Specificity: H3K27me3

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details Histone 3 ELISA Kit

Purpose: Histone H3 methylated Lys27 ELISA to quantitate the level of histone H3 lysine 27 methylation

Analytical Method: Quantitative

Specificity: Histone H3 trimethyl Lys27 ELISA Kits are tested for sensitivity in detecting trimethylated lysine 27 on histone H3 by generating a standard curve using the Recombinant Histone H3 Trimethyl Lys27 protein provided in the kit.

Components: Histone H3 trimethyl Lys27 ELISA Kits include a 96-stripwell Histone H3 Capture plate, a primary antibody specific for Histone H3 trimethyl Lys27, HRP-conjugated anti-rabbit IgG secondary antibody, Assay Dilution Buffer, 20X Wash Buffer, Developing Solution, Stop Solution, Recombinant Histone H3 trimethyl Lys27 positive control protein and plate sealer.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Recombinant protein standards are generated in duplicate from 1.56 ng - 100 ng and assayed in the kit according to the protocol listed in the manual. For lot 30114002, a developing time of 9 minutes generated the protein standard curve in Figure 1. The linear range of this lot was plotted in Figure 2. These results are provided for demonstration only. The linear range may vary depending on the developing time used and should be calculated each time a standard curve is run.

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C/-80 °C

Storage Comment: Store the Recombinant Histone H3 trimethyl Lys27 protein at -80°C. All other components should be stored at 4°C. This product is guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone h3