C3a ELISA Kit
Quick Overview for C3a ELISA Kit (ABIN2946590)
Target
See all C3a ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.78 ng/mL
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Purpose
- Pig Complement Fragment C3a (C3a) ELISA Kit
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Analytical Method
- Quantitative
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Sensitivity
- 0.47 ng/mL
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Components
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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Material not included
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Complement Fragment 3a (C3a) ELISA Kit
C3a Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Rat Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitWide Range C3a Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Sheep Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Monkey Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Rabbit Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Dog Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum
Complement Fragment 3a (C3a) ELISA KitC3a Reactivity: Rabbit Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal dilutions/concentrations should be determined by the end user.
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Comment
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The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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Plate
- Pre-coated
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Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Complement Fragment C3a (C3a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Complement Fragment C3a (C3a) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<10%
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
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Expiry Date
- 6 months
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- C3a (Complement Fragment 3a (C3a))
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Alternative Name
- Complement Component 3a
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Background
- CO3_PIG
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Gene ID
- 397072
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UniProt
- P01025
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Pathways
- Complement System
Target See all C3a ELISA Kits
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