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PDGF-BB Homodimer ELISA Kit

This Monkey ELISA Kit is a Colorimetric ELISA Kit designed to quantify Monkey .
Catalog No. ABIN2950462

Quick Overview for PDGF-BB Homodimer ELISA Kit (ABIN2950462)

Target

PDGF-BB Homodimer

Reactivity

  • 16
  • 7
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Monkey

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2 pg/mL - 2000 pg/mL

Application

ELISA

Sample Type

Plasma, Serum
  • Minimum Detection Limit

    31.2 pg/mL

    Purpose

    Monkey Platelet-Derived Growth Factor-BB (PDGFBB) ELISA Kit

    Analytical Method

    Quantitative

    Sensitivity

    18.8 pg/mL

    Components

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    Material not included

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • Application Notes

    Optimal dilutions/concentrations should be determined by the end user.

    Comment

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    Plate

    Pre-coated

    Assay Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of PDGF-BB were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of PDGF-BB were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<10%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    Expiry Date

    6 months
  • Target

    PDGF-BB Homodimer

    Alternative Name

    Platelet-Derived Growth Factor-BB
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