IDS ELISA Kit

Catalog No. ABIN3044712

Human IDS ELISA Kit Colorimetric assay for quantification of Human IDS.

Quick Overview for IDS ELISA Kit (ABIN3044712)

Target: IDS (Iduronate 2-Sulfatase (IDS))

Binding Specificity: AA 26-550

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details IDS ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IDS

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S26-P550

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <15pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Immunogen sequence: S26-P550

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Liver, kidney, lung, and placenta.

Plate: Pre-coated

Protocol: human IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S26-P550) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IDS amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IDS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IDS standard solution and each sample be measured in duplicate.

Restrictions: For Research Use only

Handling

Buffer: heparin or EDTA

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for IDS

Target: IDS (Iduronate 2-Sulfatase (IDS))

Alternative Name: IDS

Background:

Protein Function: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.

Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms: Iduronate 2-sulfatase,3.1.6.13,Alpha-L-iduronate sulfate sulfatase,Idursulfase,Iduronate 2-sulfatase 42 kDa chain,Iduronate 2-sulfatase 14 kDa chain,IDS,SIDS,

Full Gene Name: Iduronate 2-sulfatase

Cellular Localisation: Lysosome.

Gene ID: 3423

UniProt: P22304

Pathways: Glycosaminoglycan Metabolic Process