Total Antioxidant Capacity Microplate Assay Kit

Catalog No. ABIN3198900

Quick Overview for Total Antioxidant Capacity Microplate Assay Kit (ABIN3198900)

Target: Total Antioxidant Capacity

Reactivity: Various Species

Detection Method: Colorimetric

Application: Functional Studies (Func)

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate, Urine

Analytical Method: Quantitative

Product Details

Purpose: Detection and Quantification of Total Antioxidant Capacity Activity.

Characteristics: The assay measures total antioxidant capacity in which Fe3+-TPTZ is reduced by antioxidant to Fe2+ -TPTZ. The enzyme catalysed reaction products Fe2+ -TPTZ can be measured at a colorimetric readout at 593 nm.

Components: 96-Well Microplate, Assay Buffer, Substrate Diluent, Substrate, Dye Reagent, Standard, Technical Manual

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for 6 months.

Expiry Date: 6 months

Target Details

Target: Total Antioxidant Capacity

Background: Total Antioxidant Capacity, TAC, T-AOC.
An Antioxidant is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants protect the cells from damages by reactive oxygen species which are produced in oxidation reactions in the cell. Antioxidants can be small molecules such as glutathione, vitamins, or macromolecules such as catalase, glutathione peroxidase. As oxidative stress contributes to the development of many diseases including Alzheimer’s disease, Parkinson’s disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. Antioxidants are also widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline