BMP2 ELISA Kit

Catalog No. ABIN411262

Mouse BMP2 ELISA Kit Colorimetric assay for quantification of Mouse BMP2.

Quick Overview for BMP2 ELISA Kit (ABIN411262)

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Binding Specificity: AA 283-396

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Sample Type: Osseous Tissue, Cell Culture Supernatant, Serum

Product Details BMP2 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-2

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Predicted Reactivity: Bovine,Canine,Horse,Monkey,Rabbit

Sensitivity: <2pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: Q283-R396

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse bone tissue, cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse BMP-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 314, Standard deviation: 11.3, CV(%): 3.6
• Sample 2: n=16, Mean(pg/ml): 1002, Standard deviation: 39.1, CV(%): 3.9
• Sample 3: n=16, Mean(pg/ml): 2263, Standard deviation: 88.3, CV(%): 3.9,
• Sample 1: n=24, Mean(pg/ml): 334, Standard deviation: 24, CV(%): 7.2
• Sample 2: n=24, Mean(pg/ml): 1059, Standard deviation: 74.1, CV(%): 7
• Sample 3: n=24, Mean(pg/ml): 2360, Standard deviation: 177, CV(%): 7.5

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP2

Background:

Protein Function: Induces cartilage and bone formation.

Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Synonyms: Bone morphogenetic protein 2,BMP-2,Bone morphogenetic protein 2A,BMP-2A,Bmp2,Bmp-2,

Full Gene Name: Bone morphogenetic protein 2

Cellular Localisation: Secreted.

Gene ID: 12156

UniProt: P21274

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation