Nerve Growth Factor ELISA Kit

Catalog No. ABIN411337

Rat Nerve Growth Factor ELISA Kit Colorimetric assay for quantification of Rat Nerve Growth Factor and has been mentioned in 9+ publications.

Quick Overview for Nerve Growth Factor ELISA Kit (ABIN411337)

Target: Nerve Growth Factor (NGF)

Binding Specificity: AA 122-241

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details Nerve Growth Factor ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat NGF/NGF beta

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S122-G241

Cross-Reactivity (Details): There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S122-G241

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the NGF-beta family.

Plate: Pre-coated

Protocol: rat NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-G241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat NGF amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat NGF standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 84, Standard deviation: 4.62, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 366, Standard deviation: 16.84, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 628, Standard deviation: 37.68, CV(%): 6,
• Sample 1: n=24, Mean(pg/ml): 117, Standard deviation: 6.9, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 396, Standard deviation: 20.2, CV(%): 5.1
• Sample 3: n=24, Mean(pg/ml): 735, Standard deviation: 52.92, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Nerve Growth Factor ELISA Kit (ABIN411337)

Sun, Zhu, Yin, Guo, Xu, Xiao, Jiang, Guo, Meng, Lu, Wang, Peng: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).

Cai, Wu, Huang, Huang, Chen, Wang: "Effect of Zhuang Jing Decoction on Learning and Memory Ability in Aging Rats." in: Rejuvenation research, Vol. 19, Issue 4, pp. 303-8, (2017) (PubMed).

Liu, Zhang, Rong, Li, Wang: "Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron-astrocyte co-culture system through neurotrophy and neuroinflammation pathway." in: Acta pharmaceutica Sinica. B, Vol. 7, Issue 5, pp. 554-563, (2017) (PubMed).

Chung, Chung, Lee, Chung, Yang, Lee, Choi, Chang, Kim, Suh, Lee, Hwang, Do, Kim: "Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats." in: Journal of veterinary science, Vol. 17, Issue 1, pp. 97-102, (2016) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Ziv-Polat, Shahar, Levy, Skaat, Neuman, Fregnan, Geuna, Grothe, Haastert-Talini, Margel: "The role of neurotrophic factors conjugated to iron oxide nanoparticles in peripheral nerve regeneration: in vitro studies." in: BioMed research international, Vol. 2014, pp. 267808, (2015) (PubMed).

Morano, Wrobel, Fregnan, Ziv-Polat, Shahar, Ratzka, Grothe, Geuna, Haastert-Talini: "Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials." in: International journal of nanomedicine, Vol. 9, pp. 5289-306, (2015) (PubMed).

Sisman, Kiray, Camsari, Evren, Ates, Baykara, Aksu, Guvendi, Uysal: "Potential novel biomarkers for diabetic testicular damage in streptozotocin-induced diabetic rats: nerve growth factor Beta and vascular endothelial growth factor." in: Disease markers, Vol. 2014, pp. 108106, (2014) (PubMed).

Tan, Xie, Liu, Chen, Zheng, Tong, Tian: "Low-frequency (1 Hz) repetitive transcranial magnetic stimulation (rTMS) reverses A?(1-42)-mediated memory deficits in rats." in: Experimental gerontology, Vol. 48, Issue 8, pp. 786-94, (2013) (PubMed).

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background:

Protein Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI. .

Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

Synonyms: Beta-nerve growth factor,Beta-NGF,Ngf,Ngfb,

Full Gene Name: Beta-nerve growth factor

Cellular Localisation: Secreted.

Gene ID: 310738

UniProt: P25427

Pathways: Regulation of Cell Size