Myeloperoxidase ELISA Kit

Catalog No. ABIN411400

Human Myeloperoxidase ELISA Kit Colorimetric assay for quantification of Human Myeloperoxidase and has been mentioned in 7+ publications.

Quick Overview for Myeloperoxidase ELISA Kit (ABIN411400)

Target: Myeloperoxidase (MPO)

Binding Specificity: AA 49-745

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine

Product Details Myeloperoxidase ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MPO

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A49-S745

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A49-S745

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the peroxidase family. XPO subfamily.

Plate: Pre-coated

Protocol: human MPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MPO has been precoated onto 96-well plates. Standards(NSO, A49-S745) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MPO amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysate, tissue lysate, serum, plasma(heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MPO standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.076, CV(%): 3.3
• Sample 2: n=16, Mean(ng/ml): 7.4, Standard deviation: 0.4, CV(%): 5.4
• Sample 3: n=16, Mean(ng/ml): 12.4, Standard deviation: 0.72, CV(%): 5.8,
• Sample 1: n=24, Mean(ng/ml): 2.9, Standard deviation: 0.122, CV(%): 4.2
• Sample 2: n=24, Mean(ng/ml): 8, Standard deviation: 0.488, CV(%): 6.1
• Sample 3: n=24, Mean(ng/ml): 14.7, Standard deviation: 0.956, CV(%): 6.5

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Myeloperoxidase ELISA Kit (ABIN411400)

Fu, Li, Sun, Ma, Yao: "Bradykinin inhibits oxidative stress-induced senescence of endothelial progenitor cells through the B2R/AKT/RB and B2R/EGFR/RB signal pathways." in: Oncotarget, Vol. 6, Issue 28, pp. 24675-89, (2016) (PubMed).

Liu, Chen, Wu, Chen, Zhang: "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients." in: Anatolian journal of cardiology, Vol. 16, Issue 1, pp. 23-8, (2016) (PubMed).

Sousa, Morad, Feliciano, Pita, Nady, El-Hennamy, Abdel-Rahman, Cavaco, Pereira, Barreto, Leitão: "The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients." in: AMB Express, Vol. 6, Issue 1, pp. 41, (2016) (PubMed).

Chen, Yang, Liu, Teng, Ye, Xu, Cai, Cheng, Yang, Hu, Wang, Cao: "Banxia xiexin decoction protects against dextran sulfate sodium-induced chronic ulcerative colitis in mice." in: Journal of ethnopharmacology, Vol. 166, pp. 149-56, (2015) (PubMed).

Wang, Tang, Li, Wang: "Biochanin A protects against focal cerebral ischemia/reperfusion in rats via inhibition of p38-mediated inflammatory responses." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 121-5, (2015) (PubMed).

Wu, Ming, Zhang, Wu, Wu, Yao: "Edaravone rescues the lung by inhibiting lipid peroxidation and pro-inflammatory cytokines in a rat model." in: Chinese medical journal, Vol. 127, Issue 5, pp. 968-70, (2014) (PubMed).

Chen, Chen, Qin: "Effects of polysaccharides of the Euphoria Longan (Lour.) Steud on focal cerebral ischemia/reperfusion injury and its underlying mechanism." in: Brain injury, Vol. 25, Issue 3, pp. 292-9, (2011) (PubMed).

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO

Background:

Protein Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Background: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697(A49-S745) amino acids with the molecular mass of 80KDa.

Synonyms: Myeloperoxidase,MPO,1.11.2.2,Myeloperoxidase,89 kDa myeloperoxidase,84 kDa myeloperoxidase,Myeloperoxidase light chain,Myeloperoxidase heavy chain,MPO,

Full Gene Name: Myeloperoxidase

Cellular Localisation: Lysosome.

Gene ID: 4353

UniProt: P05164

Pathways: Chromatin Binding