Albumin ELISA Kit

Catalog No. ABIN454012

Rat Albumin ELISA Kit Colorimetric assay for quantification of Rat Albumin and has been mentioned in 6+ publications.

Quick Overview for Albumin ELISA Kit (ABIN454012)

Target: Albumin (ALB)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant

Product Details Albumin ELISA Kit

Minimum Detection Limit: 50 ng/mL

Purpose: The Alpha2-macroglobulin (rat)-ELISA is designed for the quantitative determination of Alpha2-macroglobulin (A2M) in rat body fluids and cell culture supernatants.

Analytical Method: Quantitative

Characteristics: The determination of A2M is carried out as competitive ELISA.

Application Details

Plate: Pre-coated

Protocol: A2M has been pre-coated onto a microplate. During incubation the binding of an anti-A2M(rat)-antibody to the wells is inhibited by A2M from samples/standards. The immobilized antibody is detected using a HRP-labelled secondary antibody. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the A2M concentration.

Assay Procedure:

Incubation of samples/standards with antibody conjugate: 50 µL + 50 µL/well 60 min, room temperature with shaking
Wash Incubation of detection antibody (HRP-labeled): 100 µL/well 60 min with shaking
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add stop solution: 100 µL/well Read at 450 nm

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for Albumin ELISA Kit (ABIN454012)

Hartner, Menendez-Castro, Cordasic, Marek, Volkert, Klanke, Rascher, Hilgers: "Tubulointerstitial De Novo Expression of the α8 Integrin Chain in a Rodent Model of Renal Fibrosis - A Potential Target for Anti-Fibrotic Therapy?" in: PLoS ONE, Vol. 7, Issue 11, pp. e48362, (2012) (PubMed).

Wenzel, Rajakumar, Haase, Geusens, Hubner, Schulz, Brewer, Roberts, Hubel, Herse, Hering, Qadri, Lindschau, Wallukat, Pijnenborg, Heidecke, Riemekasten, Luft, Muller, Lamarca, Dechend: "Angiotensin II Type 1 Receptor Antibodies and Increased Angiotensin II Sensitivity in Pregnant Rats." in: Hypertension, Vol. 58, Issue 1, pp. 77-84, (2011) (PubMed).

Hyvoenen, Saurus, Wasik, Heikkilae, Havana, Trokovic, Saleem, Holthoefer, Lehtonen: "Lipid phosphatase SHIP2 downregulates insulin signalling in podocytes." in: Molecular and cellular endocrinology, Vol. 328, Issue 1-2, pp. 70-9, (2010) (PubMed).

Hartner, Cordasic, Menendez-Castro, Volkert, Yabu, Kupraszewicz-Hutzler, Rascher, Hilgers: "Lack of {alpha}8 integrin aggravates podocyte injury in experimental diabetic nephropathy." in: American journal of physiology. Renal physiology, (2010) (PubMed).

Mansour, Mostafa, Nagi, Khattab, Al-Shabanah: "Protective effect of aminoguanidine against nephrotoxicity induced by cisplatin in normal rats." in: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, Vol. 132, Issue 2, pp. 123-8, (2002) (PubMed).

Huang, Richter, Breidenbach, Vallon: "Human C-peptide acutely lowers glomerular hyperfiltration and proteinuria in diabetic rats: a dose-response study." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 365, Issue 1, pp. 67-73, (2002) (PubMed).

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: Albumin

Background: A2M is a main part of the globulin fraction of serum. The normal serum concentration is about 20-60 µg/ml.

Pathways: Lipid Metabolism