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- Target
- Thaumatin
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Minimum Detection Limit
- 80 ng/mL
- Application
- ELISA
- Sample Type
- Food
- Analytical Method
- Quantitative
- Cross-Reactivity (Details)
- Cross reactivity: Acesufamat <0,1% Aspartam <0,1% Cycamat <0,1% Saccharin <0,1% Saccharose <0,1%
- Characteristics
- The determination of Thaumatin is carried out as direct sandwich ELISA.
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- Plate
- Pre-coated
- Protocol
- An antibody specific for Thaumatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Thaumatin present is bound.. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the Thaumatin concentration
- Assay Procedure
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Incubation of samples/standards: 100 µL/well 2 h
Wash Incubation of detection antibody: 100 µL/well 2 h
Wash Substrate incubation: 100 µL/well 15 min
Add stop solution: 100 µL/well
Read at 450 nm - Assay Precision
- Intraassay precision (CV): (n=12) at 6 ng/ml: 6,2%
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target
- Thaumatin
- Background
- The thaumatins is a mixture of proteins isolated from the katemfe fruit (Thaumatococcus daniellii Bennett). Some of the proteins in the thaumatin family are natural sweeteners roughly 2000 times more potent than sugar. Thaumatin has been approved as a sweetener in the European Union (E957), Israel, and Japan. In the United States, it is a Generally Recognized as Safe flavoring agent (FEMA GRAS 3732). The Thaumatin-ELISA is designed for the quantitative determination of Thaumatin in complex samples.
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