Anti-Mycoplasma Pneumoniae IgG Antibody ELISA Kit

Catalog No. ABIN459339

This Human ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human .

Quick Overview for Anti-Mycoplasma Pneumoniae IgG Antibody ELISA Kit (ABIN459339)

Target: Anti-Mycoplasma Pneumoniae IgG Antibody

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Plasma, Serum

Product Details

Purpose: ELISA-VIDITEST anti-M. pneumoniae IgG assay is a solid-phase immunoanalytical test.

Brand: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Characteristics: The kit is intended for diagnosis of diseases caused by bacterium Mycoplasma pneumoniae. This test is used in diagnosis of all inflammations in the respiratory system - from mild catarrh to severe pneumonia and their complications such as pericarditis, meningoencephalitis, otitis and erythema nodosum. It is recommended to monitor the dynamics of antibodies levels to improve the diagnostic value of the test. In such case, two serum samples are withdrawn from the same patient. The first sample is taken during the acute phase of the disease and the second is withdrawn ten to fifteen days later - at which time the changes of the particular antibodies level are expected. Considering the antibody isotype switching during infection, it is recommended to test all antibody isotypes (IgG, IgA and IgM) simultaneously in each sample. The strips are coated with specific antigen. The anti-Mycoplasma pneumoniae antibodies, if present in the tested sera, bind to the immobilized antigens and the antibodies being in complexes with antigen are later on recognised by animal anti-human IgG antibodies labelled with horseradish peroxidase. The labelled antibodies are revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react - a mild change in colour, if present, may be attributed to the reaction background.

Components:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 2.0 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-Mycoplasma pneumoniae IgG is a solid-phase immunoanalytical test. A specific antigen of Mycoplasma pneumoniae is bound to the surface of the wells. If relevant antibodies are present in the test samples, they bind to the immobilized antigens. In the next step, the bound antibodies then react with horseradish peroxidase-labeled anti-human IgG antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Anti-Mycoplasma Pneumoniae IgG Antibody

Target Type: Antibody