Anti-Complement Factor H Antibody ELISA Kit

Catalog No. ABIN459353

This Colorimetric ELISA kit is designed for the quantitative measurement of Human .

Quick Overview for Anti-Complement Factor H Antibody ELISA Kit (ABIN459353)

Target: Anti-Complement Factor H Antibody

Reactivity: Human

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Sample Type: Serum, Plasma

Product Details

Purpose: anti-complement factor H is an enzyme linked immunosorbent assay designed to detect IgG antibodies against complement factor H.

Brand: ELISA-VIDITEST

Analytical Method: Quantitative

Components:

• ELISA strips coated with human factor H purified from human plasma STRIPS Ag 1 x 6 strips
• 50 μL Anti-CFH IgG standard STANDARD 1 vial
• 0.1 mL IgG-HRP conjugate - detection anti-human antibody labelled by horseradish peroxidase,101x concentrated CONJ 101x 1 vial
• 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer (DB) r.t.u. 1) DIL 1 vial
• 13 mL Chromogenic substrate (TMB-BF substrate) r.t.u. (TMB/H2O2) TMB-BF 1 vial
• 13 mL Stop solution r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality control certificate 1) r.t.u. ready to use

Material not included:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: anti-complement factor H is an enzyme linked immunosorbent assay designed to detect IgG antibodies against complement factor H. The wells of the microtitrate plate are coated with purified human complement factor H. Antibodies against factor H present in serum sample bind to the immobilized factor H. Other antibodies, unbound to the factor H, are washed away during the next step. Then anti-human IgG antibodies labelled with horseradish peroxidase are added and those detect the antibodies from the sample that previously bound to factor H. The unbound labelled antibodies are washed away and the remaining labelled antibodies are visualized with a chromogenic substrate. The peroxidase activity leads to a change in colour of the solution. The reaction is stopped by adding an acidic solution. The colour intensity is directly proportional to the amount of anti-factor H antibodies in the sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Anti-Complement Factor H Antibody

Target Type: Antibody