PTH1R ELISA Kit

Catalog No. ABIN4884292

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PTH1R in Plasma, Cell Culture Supernatant and Serum.

Quick Overview for PTH1R ELISA Kit (ABIN4884292)

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 8.19-2.000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details PTH1R ELISA Kit

Minimum Detection Limit: 8.19 pg/mL

Purpose: Human PTH1R ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human PTH1R.

Sensitivity: 8.2 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Alternative Name: PTH1R

Gene ID: 5745

UniProt: Q03431

Pathways: Regulation of Carbohydrate Metabolic Process