G-CSF ELISA Kit

Catalog No. ABIN4986878

This Colorimetric ELISA kit is designed for the quantitative measurement of Human G-CSF.

Quick Overview for G-CSF ELISA Kit (ABIN4986878)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8-500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details G-CSF ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Human G-CSF Ligand

Sensitivity: 4 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: G-CSF

Background: G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced byactivated monocytes/macrophages, fibroblasts, endothelial cells, astrocytes, osteoblasts and bone marrow stromal cells. In addition, various carcinoma cell lines and myeloblastic leukemia cells can produce G-CSF constitutively. G-CSF and G-CSF receptors have also been shown to be produced by placental, decidual and endometrial gland cells during pregnancy, suggesting that G-CSF may play a role in decidual and placental functions (1). Clinical use of G-CSF has been approved for the amelioration of chemotherapy-induced neutropenia as well as for severe chronic neutropenia following bone marrow transplants. Several reviews on G-CSF and its clinical applications have been published (2 - 6).In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (aa) residue precursor proteins, have been isolated. Both proteins have a 30 aa signal peptide and have identical sequences except for a three amino acid insertion (deletion) at the 35th aa residue from the N-terminus of the mature protein. The 177 aa residue G-CSF, derived from the 207 aa residue precursor, was reported to have a ten fold lower specific activity than the shorter G-CSF isoform. Human G-CSF is 73 % identical at the amino acid level to mouse G-CSF and the two proteins show species cross-reactivity (3, 4).G-CSF exerts its biological effects through binding to specific cell surface receptors. A single class of high affinity binding sites is present in both human and mouse hematopoietic progenitors and neutrophilic granulocytes. Among nonhematopoietic cells, receptors are also expressed on placental cells, endothelial cells, and various carcinoma cell lines. cDNAs for the human and mouse G-CSF receptors have been isolated (7 - 10). Mature mouse or human G-CSF receptors are single transmembrane glycoproteins of approximately 800 aa residues. The extracellular domain of the G-CSF receptor contains three regions of homology with other cell surface proteins, including an N-terminal immunoglobulin-like region, a structural motif characteristic of the hematopoietin receptor superfamily, and three tandem repeats of the fibronectin type III domain. In humans, four alternative transcripts of the G-CSF receptor gene, encoding a soluble form of G-CSF receptor as well as three transmembrane receptor isoforms have been identified (7).

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization