IL-32 alpha ELISA Kit

Catalog No. ABIN4986954

This Human IL-32 alpha ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human IL-32 alpha.

Quick Overview for IL-32 alpha ELISA Kit (ABIN4986954)

Target: IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625-1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details IL-32 alpha ELISA Kit

Minimum Detection Limit: 15.625 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Human IL-32a Ligand

Sensitivity: 7 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-32 alpha

Target: IL-32 alpha (IL32A) (Interleukin 32 alpha (IL32A))

Alternative Name: IL-32a

Background: IL-32α is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15 kDa. Potential modifications include myristoylation and N-glycosylation. The IL-32α shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32α. Transfected IL-32α was more likely to be cellassociated as compared to IL-32β, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFNγ in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression.