CD40 Ligand ELISA Kit
Quick Overview for CD40 Ligand ELISA Kit (ABIN4987074)
Target
See all CD40 Ligand (CD40LG) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 156.25 pg/mL
-
Analytical Method
- Quantitative
-
Specificity
- Natural and recombinant Human sCD40L Ligand
-
Sensitivity
- 40 pg/mL
-
Material not included
-
- Microplate reader.
- Pipettes and pipette tips.
- EP tube Deionized or distilled water.
-
-
CD40 Ligand (CD40LG) ELISA Kit
CD40LG Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Monkey Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Chicken Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Human AA 108-261 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Human Soluble Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Mouse AA 61-260 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Human Soluble Colorimetric Sandwich ELISA 156.25-10000 pg/mL Cell Culture Supernatant, Plasma, Serum
-
-
Application Notes
- Detection Wavelength: 450 nm
-
Sample Volume
- 20 μL
-
Assay Time
- 3 h
-
Plate
- Pre-coated
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
-
- CD40 Ligand (CD40LG)
-
Alternative Name
- sCD40L
-
Background
- CD40 Ligand (CD40L), also known as CD154, gp39, TNFSF5, TRAP (TNF-Related Activation Protein) or TBAM (T-cell B-cell Activating Molecule), is a multifunctional ligand in the TNF superfamily (1-4). Interaction between CD40 and CD40L is critical to the control of thymus-dependent humoral immunity and cell-mediated immune responses (5-10). The major component of the contact-dependent signal leading to B cell activation is CD40L. CD40L stimulates B cell secretion of immunoglobulin isotypes in the presence of cytokines.CD40L is a 39 kDa, 261 amino acid (aa) glycoprotein that can form homotrimers typical of other TNFSF members (1-4, 11, 12). Proteolytic cleavage can also produce 15-18 kDa soluble forms of CD40L (13, 14). Activated T cells and platelets express both a membrane-associated and a soluble form of CD40L (sCD40L) (13, 15, 16). Platelet activation during plasma and serum sample preparation can result in artificially elevated sCD40L levels (17-20). Conversely, serum samples stored above 2-8
-
Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Target See all CD40 Ligand (CD40LG) ELISA Kits
-
