Soluble E-Selectin ELISA Kit

Catalog No. ABIN4987075

Human Soluble E-Selectin ELISA Kit Colorimetric assay for quantification of Human Soluble E-Selectin.

Quick Overview for Soluble E-Selectin ELISA Kit (ABIN4987075)

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 780-50000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Product Details Soluble E-Selectin ELISA Kit

Minimum Detection Limit: 780 pg/mL

Analytical Method: Quantitative

Specificity: Natural and recombinant Human sE-selectin Ligand

Sensitivity: 200 pg/mL

Material not included:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Application Details

Application Notes: Detection Wavelength: 450 nm

Sample Volume: 20 μL

Assay Time: 3 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for Soluble E-Selectin

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Alternative Name: sE-Selectin

Background: E-Selectin (also known as Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E) is a 115 kDa, type I transmembrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines (IL-1β or TNF-α) or endotoxins (1-4). Expression is transitory, reaching a maximum within about 6 hours of stimulation and then declining with shedding of soluble E-Selectin (1-5). Cell-surface E-Selectin is a mediator of the rolling attachment of leukocytes to the endothelium, an essential step in extravasation of leukocytes at the site of inflammation (1-6) thereby playing a key role in localized inflammatory response. E-Selectin is believed to be particularly important in inflammation involving the skin (4).The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatory-protein-like sequence (1-4). E-Selectin binds sialyl Lewis X (sLex), a sialic acid-galactose-N-acetylglucosamine-fructose tetrasaccharide, but the actual recognition is thought to be for a specific presentation of those glycosyl units in a precise three-dimensional configuration on a specific glycoprotein rather than for that particular carbohydrate (4).Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals (7, 8), probably arising from proteolytic cleavage of the surface-expressed molecule (5, 9). Elevated levels of sE-Selectin in serum have been reported in a variety of pathological conditions (8, 10-14). Although it might be anticipated that sE-Selectin would suppress leukocyte migration by competing with surface-associated E-Selectin, it may actually activate neutrophils and act as a pro-inflammatory agent (15).