C-JUN ELISA Kit

Catalog No. ABIN4993318

Product Details C-JUN ELISA Kit

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.13 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.13 pg/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AP-1 in Mouse serum, plasma and other biological fluids

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This kit recognizes natural and recombinantMouseAP-1. No significant cross-reactivity or interference between MouseAP-1 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseAP-1 and all the analogues.

Sensitivity: 46.88 pg/mL

Components: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Material not included: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Application Details

Comment:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Sample Volume: 100 µL

Plate: Pre-coated

Protocol: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AP-1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AP-1.You can calculate the concentration of AP-1in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Handling

Handling Advice: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Storage: 4 °C/-20 °C

Storage Comment: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: Transcription Factor AP-1 (AP-1) (JUN Products)

Synonyms: AP-1 ELISA Kit, AP1 ELISA Kit, c-Jun ELISA Kit, fj36h07 ELISA Kit, wu:fj36h07 ELISA Kit, zgc:65863 ELISA Kit, Junc ELISA Kit, c-jun ELISA Kit, CG2275 ELISA Kit, D-Jun ELISA Kit, D-jun ELISA Kit, D-jun/Jra ELISA Kit, DJUN ELISA Kit, DJun ELISA Kit, Djun ELISA Kit, Dmel\\CG2275 ELISA Kit, JRA ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, V ELISA Kit, cJun ELISA Kit, d-JRA ELISA Kit, d-Jun ELISA Kit, d-jun ELISA Kit, dAP-1 ELISA Kit, dJRA ELISA Kit, dJUN ELISA Kit, dJra ELISA Kit, dJun ELISA Kit, djun ELISA Kit, dm-Jun ELISA Kit, jra ELISA Kit, jun ELISA Kit, l(2)46Ef ELISA Kit, l(2)IA109 ELISA Kit, l(2R)IA109 ELISA Kit, Jun proto-oncogene, AP-1 transcription factor subunit ELISA Kit, jun proto-oncogene S homeolog ELISA Kit, jun proto-oncogene ELISA Kit, Jun-related antigen ELISA Kit, jun proto-oncogene L homeolog ELISA Kit, JUN ELISA Kit, Jun ELISA Kit, jun.S ELISA Kit, jun ELISA Kit, Jra ELISA Kit, jun.L ELISA Kit

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins