IL-33 ELISA Kit

Catalog No. ABIN5026939

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-33.

Quick Overview for IL-33 ELISA Kit (ABIN5026939)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-33 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human IL-33 concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Sensitivity: < 9.4 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-humanIL-33 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-33 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-33 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-33 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33 (Interleukin-33)

Pathways: Production of Molecular Mediator of Immune Response