Melatonin Sulfate ELISA Kit

Catalog No. ABIN5067888

Human Melatonin Sulfate ELISA Kit Colorimetric assay for quantification of Human Melatonin Sulfate.

Quick Overview for Melatonin Sulfate ELISA Kit (ABIN5067888)

Target: Melatonin Sulfate (MS)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Urine

Product Details Melatonin Sulfate ELISA Kit

Purpose: Enzyme immunoassay for the quantitative determination of melatonin sulfate in human urine.

Analytical Method: Quantitative

Sensitivity: 1 ng/mL

Characteristics: Melatonin Sulfate, Human (6-Hydroxymelatonin Sulfate, 6-Sulfatoxymelatonin) ELISA Kit

Components:

• Microtiter Plate, 1x96 wells
• PAB, 1x200ul
• Melatonin Sulfate (HRP), 40x, 1x200ul
• Standard, 0ng/ml, 1x100ul
• Standard, 1.7ng/ml, 1x100ul
• Standard, 5.2ng/ml, 1x100ul
• Standard, 15.6ng/ml, 1x100ul
• Standard, 46.7ng/ml, 1x100ul
• Standard, 140ng/ml, 1x100ul
• 0: Standard, 420ng/ml, 1x100ul
• 1: Low Control 1, 1x100ul
• 2: High Control 2, 1x100ul
• 3: Assay Buffer, 1x60ml
• 4: Wash Buffer, 20x, 1x50ml
• 5: TMB Substrate, 1x12ml
• 6: Stop Solution, 1M O4, 1x12ml

Application Details

Protocol: Principle:

• Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Melatonin Sulfate

Target: Melatonin Sulfate (MS)

Alternative Name: Melatonin Sulfate