Albumin ELISA Kit
Quick Overview for Albumin ELISA Kit (ABIN5067986)
Target
See all Albumin (ALB) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- The Mouse Albumin ELISA Kit is an in vitro immunoassay for the quantification of albumin circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.
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Analytical Method
- Quantitative
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Cross-Reactivity (Details)
- Calculated cross reactivity: Mo
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Sensitivity
- 10 ng/mL
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Characteristics
- Albumin, Mouse, ELISA Kit
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Components
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- Microtiter Plate: 1x96 wells
- Control (ng/ml), 1x650ul
- Standard (20ng/ml), 1x650ul
- Standard (50ng/ml), 1x650ul
- Standard (100ng/ml), 1x650ul
- Standard (200ng/ml), 1x650ul
- Standard (400ng/ml), 1x650ul
- HRP Conjugate (100X), 1x150ul
- Sample Diluent (20X), 1x10ml
- 0: Wash Solution (100X), 1x10ml
- 1: TMB Substrate, 1x12ml
- 2: Stop Solution, 1x12ml
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Albumin (ALB) ELISA Kit
ALB Reactivity: Human Colorimetric Competition ELISA 246.9 ng/mL - 20000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB Reactivity: Mouse Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB Reactivity: Rat Colorimetric Competition ELISA 123.5 ng/mL - 10000 ng/mL Plasma, Serum
Albumin (ALB) ELISA KitALB Reactivity: Rat Colorimetric Sandwich ELISA 3.13 μg/mL - 200 μg/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Albumin (ALB) ELISA KitALB Reactivity: Rat Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB Reactivity: Rabbit Colorimetric Competition ELISA 617.3 ng/mL - 50000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Albumin (ALB) ELISA KitALB Reactivity: Guinea Pig Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
Albumin (ALB) ELISA KitALB Reactivity: Dog Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Albumin (ALB) ELISA KitALB Reactivity: Rat Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Urine
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Protocol
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Principle:
- The Mouse Albumin ELISA kit is based on the binding of mouse albumin in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C
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- Albumin (ALB)
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Alternative Name
- Albumin
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Pathways
- Lipid Metabolism
Target See all Albumin (ALB) ELISA Kits
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